Journal: Oncology Research

Article Title: Ursolic Acid Attenuates TGF-β1-Induced Epithelial–Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5/MMPs Signaling

doi: 10.3727/096504017X15051723858706

Figure Lengend Snippet: Ursolic acid inhibits TGF-β1-induced integrin αVβ5 signaling. (A) The effect of ursolic acid on TGF-β1-induced expression of integrin αVβ5 was evaluated using Western blot analysis. Briefly, H1975 cells were treated with TGF-β1 alone or in combination with ursolic acid for 24 h. Western blotting analysis of integrin αVβ5 expression by H1975 cells. (B) Immunofluorescence revealed that H1975 cells exposed to TGF-β1 significantly increased their expression of integrin αVβ5, while ursolic acid inhibited integrin αVβ5 levels induced by TGF-β1.

Article Snippet: Ursolic acid was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA) prior to incubation with or without TGF-β1 (Roche, Mannheim, Germany) at a concentration of 5 ng/ml for 24 h. To investigate the effects of TGF-β1 or ursolic acid on cell morphology, an integrin αVβ5 inhibitor SB273005 (Selleck, Houston, TX, USA) was used at 100 nM with or without TGF-β1.

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: Oncology Research

Article Title: Ursolic Acid Attenuates TGF-β1-Induced Epithelial–Mesenchymal Transition in NSCLC by Targeting Integrin αVβ5/MMPs Signaling

doi: 10.3727/096504017X15051723858706

Figure Lengend Snippet: Integrin αVβ5 inhibitor SB273005 blocked the effects of ursolic acid on TGF-β1-induced EMT in H1975 cells. (A) Effects of SB273005 on the migration of H1975 cells with TGF-β1. A cell migration assay was performed by scratching the cell layer prior to drug treatment, and time-lapse images were obtained from 0 to 24 h. H1975 cells were treated with SB273005 (100 nM) alone or ursolic acid combined with SB273005 for 24 h in the presence of TGF-β1. (B) H1975 cells were subjected to Transwell invasion assay in the presence of SB273005 (100 nM) alone or ursolic acid combined with SB273005. Data are presented as the mean ± SD from three independent measurements. ** p < 0.01 versus control.

Article Snippet: Ursolic acid was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA) prior to incubation with or without TGF-β1 (Roche, Mannheim, Germany) at a concentration of 5 ng/ml for 24 h. To investigate the effects of TGF-β1 or ursolic acid on cell morphology, an integrin αVβ5 inhibitor SB273005 (Selleck, Houston, TX, USA) was used at 100 nM with or without TGF-β1.

Techniques: Migration, Cell Migration Assay, Transwell Invasion Assay, Control